: Name Of The Experiment:Microbiological Analysis Of Fish : Doc : Aykut Önder BARAZİ
PURPOSE:
Our aim of this experiment was to calculate the number of microorganism’s by using total plate and coliform count methods and by using swab technique.
THEORY:
Living fish carries predominantly G (-) psychotropic bacteria on their external surfaces. The internal tissues and blood system of healthy fish are usually sterile. Naturally occurring, potentially pathogenic bacteria are Clostridium botulinum especially type A and Vibrio parahaemolyticus. Fresh dish carries populations of 102 and 103 bacteria per square centimeters of skin surface or per g of gill tissue. Bacteria of genera Pseudomonas, Acinetobacter, Flavobacterium, and Vibrio are normally present in gut samples. Other G (+) such as Micrococcus, Bacillus, and Clostridium may find in small number in intestinal tract. Pseudomonas species rapidly become dominant in the micro flora of chilled fish. Acinetobacter and Flavobacterium make up the bulk of the remainder. In sanitary processing procedures can lead to contamination of seafood with potential pathogenic bacteria. Salmonella typhi is the most significant microbial contaminant. Listeria monocytogenes, campylobacter jenuni, Vibrio cholerae, Vibrio vulnificus, and V. parahaemolyticus associate with sea foods.
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