Konu: Et ve et ürünlerindeki biyojen aminler ve bunların analizleri

"Et ve et ürünlerindeki biyojen aminler ve bunların analizleri"
başlıklı ödevim var ve hiç bir sey bulamiyorum..
Arkadaşlar lütfen yardım edin..

Aydın öztanın et teknolojisiyle ilgilibir kitabı vardı.Bulabilirsen bir bak derim.

Arkadaşlar konuyla ilgili olarak size en kısa zamanda dönecektir.. Konu ilgili bölüme taşınmıştır..

bende savica adlı üyemiz gibi bu konudan muzdaripim.Acil olarak biyojen aminin analiz yöntemleri ile ilgili bilgiye ihtiyacım var.Aydınlatırsanız beni çok sevinirim.Şimdiden çok tşk ederim

ben de bulamıyorum,internette bulabildiğim bir şey yok..yardım ederseniz sevinirim...

Biogenic Amines
Biogenic amines (or polyamines) mainly include histamine, putrescine, cadaverine, spermidine and spermine. These are low-molecular, organic bases that are produced and metabolised during the normal metabolism of humans, animals, plants and micro-organisms. However, biogenic amines show a detrimental effect on the organism when taken up in large quantities. Their presence in food indicates unwanted microbial activity and, consequently, can be taken as an indicator for the degree of microbiological contamination.

In order to detect traces of biogenic amines, PICKERING offers a complete method (PCX5200 and application kit) for food and beverages. The Kit for Biogenic Amines contains, in addition to the column, reagents, diluents, eluents and a standard.

Description of the Method

The separation of biogenic amines is performed on a special potassium ion-exchange column that significantly simplifies sample preparation due to its high selectivity and robustness.
Biogenic amines are derivatised in a one-step reaction using o-phthaldialdehyde (OPA) and ThiofluorÒ (2-mercaptoethanol derivative) to a fluorescing isoindole derivative.
In the literature, different methods for biogenic amines analysis can be found describing post-column derivatisation with different types of columns and mobile solvents. Of course, the PCX5200 can be used in connection with these methods. Please ask for advice!

Chromatograms

Chromatograms of a Standard and a Contaminated Sample

HPLC Conditions and Derivatisation Parameters

Column:
ALKION® ion-exchange column for biogenic amines

Eluents:
K600 eluent and K563 eluent (potassium hydrogenphosphate buffer / isopropanol)
K130 regenerant (aqueous potassium hydroxide solution / isopropanol)

Reagent:
Solution of o-phthaldialdehyde (OPA) and ThiofluorÒ (2-mercaptoethanol derivative) in OD104 diluent (sodium borate buffer, pH-value 10.4). Concentration: 100 mg OPA and 2 g ThiofluorÒ in 1l diluent



Caution: extreme pH-range!
As a consequence of the alkaline regenerant (pH 13), components made from Vespel may not be present in the HPLC system, instead these must be exchanged for components made from pH-inert materials (Tefzel or PEEK). For advice, please contact your LC-representative or consult your technical manual.
An inert version (Titanium, PEEK) of the HPLC system is not necessary, a piston seal wash for the pump heads, however, is recommended.
To avoid corrosion of the system and contamination of the ion-exchange column with metal ions, passivation is recommended; this is especially valid for older systems. For advice, please contact your LC-representative or consult your technical manual.

Autosampler
To achieve reproducible retention times in biogenic amine analysis with ion-exchange columns, it is important to keep to exact time intervals between injections. Should only a manual injector be available, then the injection times should be controlled with a laboratory timer.

Gradient Programs

Method 05.2: ALKION® ion-exchange column for biogenic amines
Flow rate: 0.8 mls/min; Column temperature: 50°C





Chemicals and Columns

For information on chemicals and columns offered by Pickering, please refer to the page entitled Chemicals and Columns.

Literature

M. J. Walters., J. Assoc. Off. Anal. Chem. 1984, 67 (6), 1040 – 1043.
M. L. Tracy, M. V. Pickering, T. VerHulst, Food Testing and Analysis 1995, 1 (3), 48-50.
M. L. Izquierdo-Pulido, M. C. Vidal-Carou, A. Marine-Font, J. Assoc. Off. Anal. Chem. 1993, 1027 – 1032.
L. Simon-Sarkadi, W. H. Holzapfel, Z. Lebensm. Unters. Forsch. 1994, 230 – 233.