: High Performance Liquid Chromatography: Separation And Quantification Of Components In Diet Soft Drinks
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The purpose of this experiment is to quantify the caffeine content of a diet cola sample using high performance liquid chromatography (HPLC). In order to quantify the caffeine, it must be isolated from the other components in the mixture. In this experiment, you will determine a set of HPLC conditions suitable for separating caffeine, benzoic acid, and aspartame and then quantify the caffeine content of your cola sample using a standard calibration curve. At the end of this experiment you should understand the mechanisms by which components in a mixture are separated, identified, and quantified using HPLC and understand how to vary experimental parameters to optimize a separation.

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