Quality control of microbiological culture media is central to the success of the QC microbiology laboratory (USP 2004). This is reflected by recent changes in the pharmacopeia, both implemented and proposed, have increased the importance of media growth promotion (GP) studies to compendial testing (Cundell 2002, Sutton, 2005). The harmonized Sterility Test (USP 2003a) incorporates requirements for regular sterility testing of media, and the PIC/S recommendation extends this expectation to growth promotion testing of spent media (the “Stasis Test” – see PIC/S 2002). The recently harmonized Microbial Limits Tests (USP 2003b, USP 2003c) expand the requirements to an evaluation of the differential and selective properties of the media, in addition to confirming the nutritive properties. Finally, the proposed USP chapter on microbiological laboratory practices stresses the need to adequately control the growth media (USP 2004). None of these documents, however, provides detailed information on how to establish the overall quality attributes of media.

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