: Quantitative work in HPLC - Steps in Method Validation
: Pdf
: Dr. Shulamit Levin

Chemist's Concerns
Accuracy of the data
Precision or reproducibility of the data
Sensitivity of detection
Selectivity of the separation
Ruggedness of the method

Working Curve
A plot of the analytical signal (the instrument or detector response) as a function of analyte concentration, using a series of standards of known concentration.

The working curves are then used to determine the concentration of an unknown sample or to calibrate the linearity of an analytical instrument.

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