P
ART I. INTRODUCTION

1. Transgenic Wheat, Barley and Oats: Production and Characterization. . . . . . . 3
Paul A. Lazzeri and Huw D. Jones
P
ART II. TRANSFORMATION AND REGENERATION

2. Selection of Transformed Plants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Huw D. Jones and Caroline A. Sparks
3. Reporter Genes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Alison Huttly
4. Biolistics Transformation of Wheat. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Caroline A. Sparks and Huw D. Jones
5. Agrobacterium-Mediated Transformation of Bread
and Durum Wheat Using Freshly Isolated Immature Embryos. . . . . . . . . . . 93
Huixia Wu, Angela Doherty, and Huw D. Jones
6. Floral Transformation of Wheat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Sujata Agarwal, Star Loar, Camille Steber, and Janice Zale
7. Highly Efficient Agrobacterium-Mediated Transformation
of Wheat via In Planta Inoculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Thierry Risacher, Melanie Craze, Sarah Bowden, Wyatt Paul,
and Tina Barsby
8: Barley Transformation Using Biolistic Techniques . . . . . . . . . . . . . . . . . . . . 125
Wendy A. Harwood and Mark A. Smedley
9. Barley Transformation Using
Agrobacterium-Mediated Techniques . . . . . . . 137

Wendy A. Harwood, Joanne G. Bartlett, Silvia C. Alves, Matthew Perry,
Mark A. Smedley, Nicola Leyland, and John W. Snape
10. Transformation of Oats and Its Application to Improving
Osmotic Stress Tolerance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
Shahina B. Maqbool, Heng Zhong, Hesham F. Oraby,
and Mariam B. Sticklen
P
ART III. GENE AND PROTEIN EXPRESSION

11. Promoter Sequences for Defining Transgene Expression . . . . . . . . . . . . . . . 171
Huw D. Jones and Caroline A. Sparks
12. Down-Regulation of Gene Expression by RNA-Induced
Gene Silencing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
Silvia Travella and Beat Keller





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