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METHOD OF ANALYSIS
SPECTROPHOTOMETRIC INVESTIGATION IN THE ULTRAVIOLET
FOREWORD
Spectrophotometric examination in the ultraviolet can provide information on the
quality of a fat, its state of preservation and changes brought about in it by technological
processes.
The absorption at the wavelengths specified in the method is due to the presence of
conjugated diene and triene systems. These absorptions are expressed as specific extinctions
E1% (the extinction of 1% solution of the fat in the specified solvent, in a thickness of 1 cm)
1cm
conventionally indicated by K (also referred to as "extinction coefficient").
1. SCOPE
The method describes the procedure for performing a spectrophotometric
examination of fats in the ultraviolet.
2. PRINCIPLE
The fat in question is dissolved in the required solvent and the extinction of the
solution is then determined at the specified wavelengths with reference to pure solvent.
Specific extinctions are calculated from the spectrophotometer readings.
3. EQUIPMENT
3.1. Spectrophotometer for measuring extinction in the ultraviolet between 220 and 360
nm, with the possibility of reading individual nanometric units.
3.2. Rectangular quartz cuvettes, with covers, having an optical length of 1 cm. When
filled with water or other suitable solvent the cuvettes should not show differences
between them of more than 0.01 extinction units.
3.3. 25 ml graduated flasks.
3.4. Chromatography column having a length of 450 mm and a diameter of 35 mm with a
discharge tube of diameter approximately 10 mm.



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